Rat 25-Hydroxy Vitamin D (25-OH-VD) ELISA Kit

Cat.#:

JL20733

Regulatory Status:

RUO

Alternative:

calcidiol; 25-hydroxycholecalciferol;25-hydroxyvitamin D

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

3.12-200ng/mL

Sensitivity:

1.4ng/mL

Specificity:

This kit recognizes Rat 25-OH-VD in samples. No significant cross-reactivity or interference between Rat 25-OH-VD and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| 25-OH-VD

In hepatocytes (liver cells), vitamin D is hydroxylated to form 25-hydroxyvitamin D (calcidiol; calcifediol). Exposure to sunlight or dietary intake of vitamin D increases serum concentrations of 25-hydroxyvitamin D. 25-Hydroxyvitamin D constitutes the major circulating form of vitamin D, and the sum of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 concentrations in serum is used as an indicator of vitamin D nutritional status.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat 25-OH-VD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of Rat 25-OH-VD and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-115
1 : 4	Average (%)	93	98	110